what is downstream - mass spectrometry or something else? If it is MS,
then I would avoid using SDS in extraction - there are couple of methods
papers describing isopropanol/acetonitrile/water solutions for that.
I can look up more specific details, if you need.
Peter
qianxf at bu.edu wrote:
> Hello,
>> Is there somebody know how I can extract the protein from the SDS gel to the solution without digesting it?
>> Thanks a lot.
>>http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0