how to concentrate protein in SDS sample buffer

dan su dan_su at hotmail.com
Mon Mar 15 11:42:04 EST 2004

Use speedvac to vaporate your sample and then resuspend it with SDS sample 

>From: "tracy" <dreamwestus at yahoo.com>
>To: proteins at hgmp.mrc.ac.uk
>Subject: how to concentrate protein in SDS sample buffer
>Date: Mon, 15 Mar 2004 10:53:28 -0500
>I am trying to do a co-IP experiment on a HA tagged protein, and I eluted
>the protein with SDS buffer.  However, the protein level is very low that
>after I ran the eluate on a gel followed by silver staining, I couldn't see
>any band.  So now I am thinking combining all the eluate from multiple
>experiments.  Is there a way that I can concentrate all these proteins?
>Will TCA precipitation work in the presence of SDS?  Or is there any kind 
>concentrator that I can use?  Thank you very much!

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