I am currently working on the most difficult project I have ever seen and have finally been forced to take the plunge and ask for outside assistance.
This protein is a novel type from bacteria. Size exclusion chromatography suggests it to be 35-45kDa. It does not stain by coomassie, silver stain or any traditional techniques on SDS-PAGE. However, it is stained by Stains-All, but this shows it to be stuck at the stacking/resolving gel interface.
We have tested for amino-sugars and found none.
Does anyone have any idea why a protein would not travel through a polyacrylamide gel at all?
If anyone has noticed anything like this before I would love to speak with you in more detail.