I am trying to resolve a fluorophore-conjugated peptide mixture on a
Beckman CE using an uncoated capillary. So far I have been using a
25mM phosphoric acid buffer system (pH 2.5),
but while I do see nice peaks using LASER-induced fluorescence, the
patterns are in no
way reproducible, even from run to run. Does anybody have a good guide
to CE trouble-shooting, or - better yet - a fool-proof protocol?
Ralph Peteranderl, Ph.D.