Peptide capillary electrophoresis

Ralph Peteranderl rpeteranderl at partners.org
Tue Mar 2 17:15:46 EST 2004

I am trying to resolve a fluorophore-conjugated peptide mixture on a 
Beckman CE using an uncoated  capillary. So far I have been using a 
25mM phosphoric acid buffer system (pH 2.5),
but while I do see nice peaks using LASER-induced fluorescence, the 
patterns are in no
way reproducible, even from run to run. Does anybody have a good guide 
to CE trouble-shooting, or - better yet - a fool-proof protocol? 

Ralph Peteranderl, Ph.D.


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