white out

David Cavanagh david.cavanagh at ed.ac.uk
Wed Jun 30 07:23:15 EST 2004

msd at ior.twmu.ac.jp (??) wrote in message news:<BD0761EB.894%msd at ior.twmu.ac.jp>...
> Hello.
>  My problem is the 32kDa protein is supposed to be there,
> and other experiments, RT-PCR, IHC, and DNA ladder assay
> suggest the exhistance of the protein, but the objective band
> in western blot , though it is supposed to be black on X-ray
> film,  is a white band just like a ghost in the night. Is there anyone
> who has the right method to avoid this failure?  I used  goat IgG
> for first antibody, and second antibody was Rabbit IgG anti
> goat IgG.    Please Help me!

Too much HRP, I believe, although some people say that too much
primary Ab can produce the same effect





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