Quoting "$BB52,(J" <msd at ior.twmu.ac.jp>:
>>> My problem is the 32kDa protein is supposed to be there,
>> and other experiments, RT-PCR, IHC, and DNA ladder assay
>> suggest the exhistance of the protein, but the objective band
>> in western blot , though it is supposed to be black on X-ray
>> film, is a white band just like a ghost in the night. Is there anyone
>> who has the right method to avoid this failure? I used goat IgG
>> for first antibody, and second antibody was Rabbit IgG anti
>> goat IgG. Please Help me!
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003