Help !!!!
I am expressing my protein in E. coli BL21, and I am looking for
another way to disrupt the cells without sonication
Does anyone have
a protocol ?!?!?! I tried lyzosyme followed by frezze-defrezze
methodology, but the separation of pellet, supernatant and debrit was
terrible
I needed to centrifugate several times, so I am not sure
about the integrity of my protein
I am growing a culture of 50 mL.
Please, send me some advice !!! Thanks everybody,
Claire
