Membrane seperation

[James Wee]

Hello,

I tried to obtain the Pseudomonas outer and cytoplasmic membrane using both
R.E.Hancock, H.Nikaido
(http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=218670) and
N.C.Jones, M.J.Osborne procedure. However, I could not get the membrane
fraction as described. Does anyone has any new procedure or techniques?

Thanks,
James