IUBio

mysterious lysozyme or what?

kaj.stenberg at helsinki.fi.invalid kaj.stenberg at helsinki.fi.invalid
Thu Jan 29 03:52:15 EST 2004


P.C. <PC at no.email.sorry> wrote:
> Kaj, which pH did you use for the Q and SP sepharoses (for lysozyme)?

> lysozyme has several disulfides; depending on Cys reduction/oxydation 
> status and pH, the protein might bind to anion echange and elute in 
> several peaks. With all its Cys ingaged in disulfides the pI will be 
> above 10!!

Both were run with 20mM Tris-2mM ZnCl2, pH 7.5. B buffer had 1M NaCl.

-- 
Kaj




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