On 11 Jan 2004 15:16:36 -0000, viegas at embl.de wrote:
>>Hi!
>I wonder if someone has a protocol for [35S]methionine incorporation to be used
>in Hela cells. In particular, I am interested in the amount of uCi to use per
>ml. and per number of cells. Secondly, I´d like to now if the cells have to be
>in confluence for this essay or not. Thank you very much in advance,
> Marcelo Viegas
>
I've used as few as 100-200 cells, or up to 10 million. And from 5 uCi
to over 200 uCi of [35S]methioine. It depends on what you are trying
to do. Are you looking at general labelling of proteins, or trying to
immunoppt. a specific (perhaps rare) protein?
Nick
--
Nick Theodorakis
nick_theodorakis at hotmail.com
nicholas_theodorakis [at] urmc [dot] rochester [dot] edu
