protocol for methionine incorporation

[viegas at embl.de]

Hi!
I wonder if someone has a protocol for [35S]methionine incorporation to be used 
in Hela cells. In particular, I am interested in the amount of uCi to use per 
ml. and per number of cells. Secondly, I´d like to now if the cells have to be 
in confluence for this essay or not. Thank you very much in advance,
                                        Marcelo Viegas

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