potein expression

Tom Knight tk at suspiria.ai.mit.edu
Mon Jan 5 09:11:26 EST 2004

I assume you are adding IPTG to your medium.  The pET vector series
has a T7 promoter, which will normally not be recognized by E. coli
polymerases.  Only when the T7 polymerase is expressed will your gene
be expressed.  The T7 polymerase is under control of a Lac promoter,
which is positively regulated by the addition of IPTG.

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