Alternatives to Laemli?

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Wed Feb 18 07:40:42 EST 2004

ahernan at cica.es wrote:

> Hi all,
> I am trying to run a protein-RNA complex on a SDS-PAGE gel but I was 
> told that laemli's high pH can promote RNA hydrolysis. Any one knows 
> of an alternative buffer system for protein SDS-PAGE at (near) 
> neutral pH? I know Novex sells them as precast (and so do BioRad and 
> Amersham...so it must not be under a patent) but buying such 
> expensive stuff at this stage is out of the question.

Jovin has tested a large number of buffer systems in silico for
Disk-electrophoresis (Ann. N.Y. Acad. Sci. 209 (1973) 477-96 and
references therein).

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