ahernan at cica.es wrote:
> Hi all,
>> I am trying to run a protein-RNA complex on a SDS-PAGE gel but I was
> told that laemli's high pH can promote RNA hydrolysis. Any one knows
> of an alternative buffer system for protein SDS-PAGE at (near)
> neutral pH? I know Novex sells them as precast (and so do BioRad and
> Amersham...so it must not be under a patent) but buying such
> expensive stuff at this stage is out of the question.
Jovin has tested a large number of buffer systems in silico for
Disk-electrophoresis (Ann. N.Y. Acad. Sci. 209 (1973) 477-96 and
references therein).
