IUBio

Alternatives to Laemli

Joe Doe none at mail.utexas.edu
Tue Feb 17 18:46:05 EST 2004


In article <f05210601bc5800da3305@[193.146.54.140]>, ahernan at cica.es wrote:

> Thanks to all,
> 
> I think I'll try Mike Sullivan's suggestion but with a stacking on 
> top (maybe 3% ).
> As for the presence of SDS, the protein and the RNA are (hopefully!) 
> crosslinked, so, if there are no problems with size, the complex 
> should enter the gel and stay put.
> Anyone heard about a Tris-Acetate system, anyway?
> Cheers
> 
> Agustin



Search for Jovin buffers (span the entire pH range).

You can find more information in the book: Gel electrophoresis a practical
approach.

Roland

> ---



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