expression periplasm

M Sin esineva at netscape.net
Mon Feb 16 16:04:39 EST 2004

Refolding, 18kDa- should not be problem.

"jocelyne matgelin" <jocelyne.mathelin at u-bourgogne.fr> wrote in message
news:9e43adef.0402130550.111ec4d1 at posting.google.com...
> I try to espress a 18KD protein (from calycins family) in pET-22b
> vector with BL21(DE3) strain, in order to obtain the protein of
> interest in the periplasmic space (for disulfide bonds formation).
> After several esperiments, I obtain my protein only in inclusion
> bodies.
> I tried different temperatures, with or without induction with IPTG at
> different concentrations, short or long time culture.
> What can I do now?
> Thanks

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