> Hi all,
>> I am trying to run a protein-RNA complex on a SDS-PAGE gel but I was
> told that laemli's high pH can promote RNA hydrolysis.
and SDS?? - is your complex stable in SDS?
> Any one knows of
> an alternative buffer system for protein SDS-PAGE at (near) neutral pH?
a 3 - 10 % PAGE in TBE buffer will probably work in your case. Check the
literature on EMSA ("gel-shifts") any of the standard buffer system will
be a good place to start.
> I know Novex sells them as precast (and so do BioRad and Amersham...so
> it must not be under a patent) but buying such expensive stuff at this
> stage is out of the question.
so for many of us... Try TBE.
> Thanks a lot.
> Cheers,
> Agustin
> ---
