I am trying to run a protein-RNA complex on a SDS-PAGE gel but I was
told that laemli's high pH can promote RNA hydrolysis. Any one knows
of an alternative buffer system for protein SDS-PAGE at (near)
neutral pH? I know Novex sells them as precast (and so do BioRad and
Amersham...so it must not be under a patent) but buying such
expensive stuff at this stage is out of the question.
Thanks a lot.