I was also going to suggest gel filtration as an alternative, but then I
realized that the problem you may be having is that the on-off rates may
be causing your complex to get separated to bits in PAGE and probably
would have the same problem in gel filtration. How do you detect your
proteins? Are you just starting with two purified proteins and seeing if
they bind to each other or is it a complex mixture like a cell lysate?
Lou Hom >K'93
lhom at ocf.berkeley.eduhttp://www.ocf.berkeley.edu/~lhom/