I am currently working on a project showing the enhanced binding of two proteins via site directed mutagenesis and would like to visualize this by Native Gel. I have made a poor attempt at this using a protocol from Sigma-Aldrich on a 12.5% pre-cast Bio-rad gel and my proteins completely separated on the gel. Are there any protocols out there that I could use that would help me with this, perhaps by lowering the ionic strength of the running and/or sample buffers.
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
