miaoqf at sina.com miaoqf at sina.com
Mon Feb 9 09:43:31 EST 2004

I expressed a protein in E.coli sucessfully  and the DNA sequence is right. The theoretical MW is 25KD. But after its purification, the MW is 29-30KD in a reducing-SDS PAGE. More surprised me is the MW is 25KD when I run it in a non-reducing SDS pAGE. I don't know the reason. Who can help me ?


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