Dr Engelbert Buxbaum wrote:
>> Note that since proteins crystallised are often produced in bacteria by
> genetic engineering, and bacteria do not clip,
Not true.
Hirel et al, Proc Natl Acad Sci U S A. 1989 Nov;86(21):8247-51.
Extent of N-terminal methionine excision from Escherichia coli
proteins is governed by the side-chain length of the penultimate amino
acid.
> crystal structures
> (PDB-fiels) often contain the complete N-terminus. I have often wondered
> how this might affect the result...
