Principles of Columns & Protein Purification

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Fri Aug 13 04:59:50 EST 2004

Tin Cup wrote:

> Hi All:
> Can you please advise where would a newbie start to read from for Protein
> purification and principles of columns.

Any book on biochemical lab techniques, eg. Cooper or Richter, will
cover these topics. Several volums of "Methods in Enzymology" deal with
protein purification and give a good account of the various techniques.

Also very useful are the booklets on protein chromatography and
electrophoresis published by the company Pharmacia. Although written to
promote their products, these booklets contain a lot of generally
applicable information. Last time I looked, they had them as pdf-files
on their web site.

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