I guess one could use "homogenizers" that one could buy (Bio Rad?),
sonication could work, buffer to use would depend on intended use of the
Rolands G. Aravindan
rolands_aravindan at yahoo.com
Scott Coutts wrote in message <3F435ED3.6060601 at med.monash.edu.au>...
>Oh, sorry, I forgot to mention something... I cant remember what the
>little eppy type mortar and pestles were called or who sold them!
>>What about sonication?
>>Scott Coutts wrote:
>> Hmm... dont know about the BEST way, but you can buy little 'mortar and
>> pestle' type things that fit exactly into the bottom of an eppendorf
>> tube. We used them a while back for crushing and homogenising individual
>> Drosophila flies.
>>>> As for the solvent, well, I guess that depends on how you have your
>> ELISAs set up.
>>>>julie.decock at med.kuleuven.ac.be wrote:
>>>>> what is the best method for homogenization of very small
>>>> > amounts (2-18 mg) of mammary tumor tissue for ELISA and
>> > in which solvent should this be done?