just curious: did you load the samples while they were still hot? (which
is the most common way to deal with low solubility of Gu- or
K-dodecylsulfate).
Peter
ss at nordicbioscience.com wrote:
> I tried to analyse by western-blot protein samples that I have treated with guanidium. But the proteins migrated in a smear, like if all the samples were degraded. Is there a problem to run an SDS page in presence of guanidium?
>>>>http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0