> Hi, all
>> I am planning to do a co-IP on an inner membrane protein with HA tag of
> Caulobacter(a gram negative bacterium), and I am hoping to pull down some
> protein complexes under non-denaturing condition (low detergent). Has
> anybody done similar experiments like this? I really don't know where to
> start, does anybody have a protocol for that?
>> How shall I determine the right condition that can solubilize the membrane
> protein yet maintain the complexes? For HA tag, which antibody will be
> better, monoclonal or polyclonal?
Calbiochem offers heat-killed protein A (Pansorbin) or protein G
containing bacteria for immunoprecipitation experiments, and they have
published a little booklet on methods. You may wish to consult this.
Usual disclaimer applies.