We are trying to purify DPPIV from drosophila using standard techniques
from the lit. We are finding that after putting the homogenate of
Membrane fraction through a DEAE column, and concentrating we lose all
the enzymatic activity. We were able to concentrate after gel
filtration, and there is activity in the fractions off the DEAE column.
Does anyone have any suggestions as to where to look for the problem?
Thanks.
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