Hi just wondering if anyone had some ideas for this
Q/ You want to prepare a quantity of a protein from a biological sample. The protein has a MW of about 45kD and a pI of 6. It binds to the amino acid tyrosine but in the crude biological sample the protein is already saturdated with tyrosine, and the protein is unstable below its isoeletric point. Outline a scheme, by which the protein could be purified by chromatographic means..including stationary phase, and conditions for elcution etc
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
