On 11 Mar 2003, Phil Harrison wrote:
> Worse news! I tried redissolving the goo, including several spots on the
> side of the freeze-dryer flask, and it didn't go into solution. All I got
> was a cloudy suspension. Any thoughts? Have I removed something (a
> carbohydrate--maybe a sugar alcohol) that was keeping the protein from
> becoming fully dehydrated?
>> Thanks,
>> Phil
>> Phil Harrison
>> USDA-Agricultural Research Service,
> Forage and Range Research Lab
> Utah State University, UMC 6300
> Logan, UT 84322-6300
> Phone: 435-797-3209
> FAX: 435-797-3075
> e-mail: arsphys at cc.usu.edu>> ---
>
What is the buffer comnposition of your biogel column?
Have you tried dialyzing your column fractions against a volatile solvent
(like 0.1% acetic acid...BTW, what is the pI of your protein) prior to
lyo?
JIm
