On 20 Mar 2003 sandrine.vessillier at caramail.com wrote:
> Hi,
> I actually try to purify 2 proteins by gel filtration, one is 25KDa and the other one 15 KDa. SDS PAGE analysis indicates the first pic to be eluted is the 15KDa protein and I can not see the 25 KDa protein. Do you think it is retained on the column ?(I use 6M guanidine and 0.5M NaCl in tris buffer pH8 for loading and separation). Do you have some suggestions?
>>>>http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0>
Superdex resins often require "conditioning". Try running your sample
over and over the column a few times.
Also, how do your monitor the elution from the column?
Jim
