On Wed, 05 Mar 2003 09:59:33 +0100, Dr Engelbert Buxbaum
<engelbert_buxbaum at hotmail.com> wrote:
>fisher wrote:
>>> If I want to get this kind of protein in E.coli, how to do it?
>> Does it need in vitro translation?
>>>Buy some Translabel (a mixture of 35S methionine and cysteine) and a
>sulfur-free medium (for mammalian cell culture these are readily
>available, I'm not sure for E. coli). Grow your bugs in an o/n culture,
>spin them down and wash with sulfur-free medium.
...
Is TransLabel cheaper than [35S]sulfate? I thought that TransLabel was
made by incubating bugs in [35S]sulfate and then taking an amino acid
fraction.
Nick
--
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu
