BTW, ar you sure Lowry's needs 750nm? I haven't used Lowry in a long time, but
if my memory serves me right, the absorbance is measured at around 600nm!
Quoting James Wee <jywee at charter.net>:
> I use OD 750 to read cellular protein concentration of a bacteria. I don't
> use Coomassie reagent, instead, I use Folin reagent as the stain. I use 30,
> 60, 90 and 120 µg of BSA to construct the standard curve. It doesn't sound
> right to substract 0.248 from all sample readings. Some of the sample
> readings will go below 0, though the curve goes through 0. Could u explain
> more about over the linear range of detection?
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003