I use OD 750 to read cellular protein concentration of a bacteria. I don't
use Coomassie reagent, instead, I use Folin reagent as the stain. I use 30,
60, 90 and 120 µg of BSA to construct the standard curve. It doesn't sound
right to substract 0.248 from all sample readings. Some of the sample
readings will go below 0, though the curve goes through 0. Could u explain
more about over the linear range of detection?
Thanks,
James
