no bands in western blotting!!!!!!!!!!!

EK anybody at uchicago.edu
Wed Jun 25 08:48:52 EST 2003

<cvaravindhan at yahoo.co.uk> wrote in message
news:20030625122256.8089.qmail at ww02.hostica.com...
> hi guys,
> i am working with a protein which is 10kda.i am not able see a band at the
expected region in westernblotting-things which i hav used
> 1.amersham ECL NCM -poresize 2u
> 2.0.1%SDS in blot buffer
> 3..63A for 30-45 min
> 4.0.3% Tween-20 in PBS for wasing and blocking
>  plz suggest me few things abt were i am going wrong.

First thing I would try is loading more protein on the gel. Then, higher
concentration of the antibody. Do you see other proteins being transferred
to the membrane? You can quickly stain/destain the membrane using SDS-PAGE
Coomassie staining solution. If you don't see protein bands, that means the
transfer did not happen (wrong membrane?). Usually, TBS or PBS with 0.1%
Tween-20 works fine. 5-50 ng of protein per lane, 1/10000-1/500 dilution of
0.2 mg/ml antibody are the usual working ranges. One more IMPORTANT thing in
case nothing works: your antibody may recognize the protein in native,
folded state and would not recognize the denatured, linear protein.
Solution: try another antibody (e.g., one raized against terminal peptide
sequences of the protein). As a last resort, in case another antibody is not
available, you may try renaturing the protein right on the membrane (depends
on whether your protein is a monomer in nature). There must be methods for
doing this somewhere out there, although I never tried it myself. Generally,
you would do the same thing as for in-solution renaturation.

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