hi ,
try 2.5% final conc. of dodecyl maltoside and spin the sample shortly
before loading on gel.
bye
rajeev
Ben Long wrote:
> Hi all,
>> I am trying to run blue native gels of cyanobacterial thylakoid membrane
> complexes and keep running into a problem with protein precipitation in the
> gel. I am extracting with 1.5 % dodecyl maltoside and running gels
> according to the method of Schagger et al. If anyone has developed new
> methods or managed to trouble-shoot this sort of thing before I would be
> interested to know how and what can be done to achieve nice band separation
> and reduced precipitation.
>> Cheers,
>> Ben
>>
