I need to radioiodinate a protein to use in immunoprecipitation assays
for screening hybridomas producing MAbs specific to this protein of
interest. I will be purchasing recombinant, carrier-free protein for
this purpose and intend to have it commercially labeled.
I have never worked with radioiodinated proteins and therefore have
some basic questions, such as: (i) will the structure and/or stability
of the protein be affected by this process (and if so to what
degree...what properties of the protein could predispose to structural
changes/instability following radioiodination)?; (ii) given the
half-life of I-125 (60 days?), at what point in time following
labeling should I expect to see a decline in radioactivity to the
point that sensitivity for the purposes of immunoprecipitation would
be "lost"?
Thank you for your time, if anyone could help me directly with finding
answers or point me in the right direction, it would be much
appreciated.
Kiley
