Hi all,
I am trying to run blue native gels of cyanobacterial thylakoid membrane
complexes and keep running into a problem with protein precipitation in the
gel. I am extracting with 1.5 % dodecyl maltoside and running gels
according to the method of Schagger et al. If anyone has developed new
methods or managed to trouble-shoot this sort of thing before I would be
interested to know how and what can be done to achieve nice band separation
and reduced precipitation.
Cheers,
Ben
