I would like to get some ideas for exptl strategies that can be used to determine whether endocytosis of a given protein is required for its biological effect on cells. I already know this protein has cellular effects, I also know that it is endocytosed by adherent cells fairly quickly, in a saturable manner, and that about 30% of this uptake is specific. I need some approaches for testing a link between uptake and cellular effects. I've already got some ideas, and have some studies going using transfection of a dominant negative mutant for dynamin, but hopefully someone who has some experience with this can help with some other ideas--simple, complicated, old school or new technology, I don't care; at this point anything might help. Thanks.
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
