protein localization

yuancan at vip.sina.com yuancan at vip.sina.com
Tue Jun 10 08:40:29 EST 2003

I insert ORF of my gene into pEGFP-N1 to determine the protein localization. But there is no signal of fluorescence can be detected. And there is strong signal of fluorescence with the empty vector. The inserted cDNA include initial ATG and without a stop codon. The sequencing result shows no mutant and correct reading frame. Who can tell me if there is any element I have neglected?


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