which Tag is best?

Patrick bier13 at mail.com
Wed Jun 4 07:23:59 EST 2003

jdu at benaroyaresearch.org wrote in message news:<20030602225342.30221.qmail at ww02.hostica.com>...
> I want to put a tag at either end of a gene of interest, and then
express this gene in Mouse T cells. Finally, I want to recover the
expressed fusion protein with the monoclonal antibody against this
tag. I do not want my fusion protein to be contaminated with other
proteins from T cells (I mean the monoclonal antibody has negligible
cross reactivity with other proteins from T cells). Which tag is the
best for this purpose? Please help me make this decision? Thanks.
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0

We use flag and HA tag, both work well. The flag tag is the one we use
most of the time.


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