regarding tissue culture growing problem

Dr Engelbert Buxbaum engelbert_buxbaum at web.de
Tue Jun 3 05:47:11 EST 2003

raj_anand123 at rediffmail.com wrote:

> For research purpose I am culturing human cells. When the cells  are
> seeded in the falcon tube it growing nicely but when I seeded in to  96
> well plate it does not growing. So is there any remedy or your
> experience?What could be the reason? 

Are the wells treated for tissue culture? Cells do not adhere to
standard plates.

Are you using the same medium and growth conditions in tubes and plates
(temperature, humidity, CO2 level)?

When you ceck the cells under the microscope, have they attached to the
bottom of the wells and spread out, or are they rounded and float in the

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