Historians believe that in newspost <b16hhp$d92$1 at news.Stanford.EDU> on
Tue, 28 Jan 2003, meb <meb at meb.emb> penned the following literary
masterpiece:
>your reply couldn't have been more helpful.
Well that is your pig ignorance in not choosing to learn how to do a
search properly and asking a pretty vague question. You then shoot down
a genuine reply. If you had mentioned pET vectors all would have been
revealed.
>have you actually used phage to
>induce expression
Yes. CE6. If you know about T7 RNA polymerase expression vectors you
would know about Studier, Tabor or Richardson. A search on them could
bring great rewards, especially as the system is I think licensed, if
not patented.
>or do you just need to reply to everything?
Well given that I rarely, if ever, post this group (14x since 1993
according to googlegroups) that is bollocks. I tend to post to the
methods reagents newsgroup, where an archives search would show whether
I talk crap or not. If you searched the archives of
bionet.molbio.methds-reagnts00 you would find a lot of posts on CE6
including three from me with the first one dating back to 1996.
>I am curious about how well phage induction of vectors like the pet series
>works for expression of poorly tolerated constructs.
Try Novagen, Stratagene plus a search for CE6 would fined the
appropriate papers.
>
Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
Duncan Clark
GeneSys Ltd.
