IUBio

phage induction

Peter Cherepanov peter.cherepanov at uz.kuleuven.ac.be
Wed Jan 29 03:32:13 EST 2003


AFAIK, the phage that is always used for that is called CE6.

Doherty et al, Overproduction of the toxic protein, bovine pancreatic
DNaseI, in Escherichia coli using a tightly controlled T7-promoter-based
vector. Gene-1993, 136, 337-340.

Miao et al, Characterization of gene expression in recombinant Escherichia
coli cells infected with phage lambda. Biotechnol Prog-1993, 9, 153-159.

Wu and Santi, High level expression and purification of HhaI
methyltransferase.
Nucleic Acids Res-1988, 16, 703-717.

+ Stratagen's kit protocol.


Peter


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