your reply couldn't have been more helpful. have you actually used phage to
induce expression or do you just need to reply to everything?
I am curious about how well phage induction of vectors like the pet series
works for expression of poorly tolerated constructs. It is not often used
anymore. I have indeed tried google and pubmed (which was a truly inspired
suggestion), but keywords like phage and induction pull up too many hits to
zero in on anything approaching a useful protocol.
Are there particular papers amongst the umpteen you allude to that could be
useful?
"Duncan Clark" <junk@[127.0.0.1]> wrote in message
news:kSWhF7YAGsN+EAdu@[127.0.0.1]...
> Historians believe that in newspost <b146vm$2vk$1 at news.Stanford.EDU> on
> Mon, 27 Jan 2003, Mark Bowen <mboNOT at slac.stanford.edu> penned the
> following literary masterpiece:
> >Specifically introduction of T7 polymerase by phage. Does anyone have
> >any experience in this area?
>> Umpteen old papers on T7 around.
>> What do you actually want to know and have you tried Pubmed or Google
> etc. ?
>> Duncan
> --
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
>> Duncan Clark
> GeneSys Ltd.
