Hello,
I'm running 2D Electrophoresis and after Silver staining the gels, I observed few proteins.
The problem is that proteins do not transfer from the strip to the gel during second dimension, they stay in the strip.
I try longer equilibration times and other IEF methods without success.
Does anybody know how to solve this problem?
thanks,
Maria Codevilla
Proteomic Division
GEMA Biotech
Buenos Aires
Argentina.
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
