poor protein transfer from strip to gel

[mcodevilla at gemabiotech.com]

I'm running 2D electrophoresis and after staining the gel, I see few spots. The problem is that proteins stay in the strip during the second dimension. 
I 've already try longer equilibration times and other IEF methods without success.
Does anybody know how to solve this?

thanks,
Maria Codevilla
Proteomic Division
GEMA Biotech
Buenos Aires
Argentina.



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