Hi,
I am using Ni affinity column to isolate my protein which came out
successfully with Imidazole. However, I found a lot (~100 mg) of protein
still sticking to the column and I eluted them out by stripping off the
Ni+2 from the column using EDTA. So, this solution now contains my protein
+ (may be some contaminant) + Ni and EDTA and is colored blue (as
expected). At this point I wonder if there is any way / standard practice
to separate the protein from Ni+2, so that I can re-utilize it.
Any help would be appreciated.
Regards,
Deb
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Debashis Mukhopadhyay, Ph.D.
# /\_/\ Research Associate, Div of Cellular Biology
(( / o o \ Dept. of Molecular & Experimental Medicine
\\ \~(*)~/ The Scripps Research Institute
\/ `-' 10550 N. Torrey Pines Rd
| \|| || Mail drop: MEM 116
\ '|| || La Jolla, CA 92037
\)()-())
Tel: (858)-784-7944/7943 "8^>)
Fax: (858)-784-7966
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