On 13 Jan 2003 mrsankar_in at yahoo.co.uk wrote:
> I have a His Tag protein overexpressed in E.coli and it does not go into inclusion bodies. I want to crystallize the protein for which i need it in a very high concentration and in very pure form. What way i can purify the protein in Ni Column? Denaturing or native conditions?
>What info do you hope to gain from a crystal of denatured protein?
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legatek at mcmaster.ca Kyle Legate legatek at hotmail.com
Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
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