> > I'd like to try my hand at simultaneous primary antibody incubations
> > in my Western immunoblots. My bands are separated sufficiently in a
> > 12% gel, and stripping doesn't seem to work. So I figured I'd just
> > stain for both the protein of interest (SNAP-25) and a housekeeping
> > protein (B-actin) at the same time. I have heard from others on this
> > group that many people do this, but does anyone have a good paper they
> > can use to site this method? I've tried to find one from Pubmed and
> > Ovid but I'm coming up a bit blank.
Hi,
I've seen (I can't remember where!) a system where you incubate with
two fluorescent secondary antibodies and are then scanned using a
phosphoimager.
Nick
