IUBio

double immunoblotting

Esk sophiemalpass at hotmail.com
Sat Jan 11 13:27:47 EST 2003


On Fri, 10 Jan 2003 21:11:05 +0000, Jason Wong wrote
(in message <a07dedf1.0301101311.6761385e at posting.google.com>):

> I'd like to try my hand at simultaneous primary antibody incubations
> in my Western immunoblots.  My bands are separated sufficiently in a
> 12% gel, and stripping doesn't seem to work.  So I figured I'd just
> stain for both the protein of interest (SNAP-25) and a housekeeping
> protein (B-actin) at the same time.  I have heard from others on this
> group that many people do this, but does anyone have a good paper they
> can use to site this method?  I've tried to find one from Pubmed and
> Ovid but I'm coming up a bit blank.

test (sorry)




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