I'd like to try my hand at simultaneous primary antibody incubations
in my Western immunoblots. My bands are separated sufficiently in a
12% gel, and stripping doesn't seem to work. So I figured I'd just
stain for both the protein of interest (SNAP-25) and a housekeeping
protein (B-actin) at the same time. I have heard from others on this
group that many people do this, but does anyone have a good paper they
can use to site this method? I've tried to find one from Pubmed and
Ovid but I'm coming up a bit blank.
